Meniscus formation matrigel?

Hey guys, I am currently doing tube formation assay on matrigel coated coverslips, and i wonder if there is any possibility to get rid of the meniscus formation? I have only a small part of the...

09 February 2021 6,979 3 View

Any research on modeling skew Gaussian spatial data in the presence of censored data?

Any research on modeling skew Gaussian spatial data in the presence of censored data?

15 December 2018 4,284 3 View

Are Gomori buffers prepared in the ratios given or do they require additional titration to obtain the correct pH?

I have been trying to prepare some buffers for pH assays in the range of 1-11. Starting with pH1, I noticed that mixing the solutions as shown in the table does not give the correct pH. Instead,...

24 February 2015 4,553 4 View

How long do you use Versene to detach adherent hepatocyte cell line (for further facs analysis)?

17 February 2014 2,346 2 View

What's the most relevant prostate cancer animal model?

In order to test nutrition or physical activity, I would like some advice on the best prostate cancer animal model: orthotopic or subcutaneous, human or rat or mouse?

04 December 2013 3,492 7 View

Difference between HBEC and 16HBE?

Do the mRNA expression profiles of primary HBEC culture differ much from cultured 16HBE cells? (In terms of WHICH mRNAs are expressed)?

10 November 2013 3,026 1 View

BLAST Search Exclusions?

Is there a way of excluding plus/minus strand results in a nucleotide BLAST search?

10 November 2013 2,952 1 View

What is the role of poly-L-ornithine found in a protocol for plate coating with bovine fibronectin?

It seems to be becessary for neuronal cell culture, is it for other cell types?

07 October 2012 3,898 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Blast analysis of RC,UHPC slabs using material model *MAT_72R3 in LS-DYNA?

20 February 2021 5,984 3 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View

Useful architecture for bioinformatics?

Hi Dear researchers In ensemble-based architectural design Which algorithms are more useful for classification? What is the difference between parallel and ensemble architecture? Thanks I am...

08 February 2021 2,727 5 View

Electron density difference maps(Fo-Fc): what is the meaning of negative densities (red blobs)?

Dear community, As far as I understand, negative density features point at stuff that is in the model, but not supported by experimental data. However, in many cases (e.g. attached image), when...

06 February 2021 2,847 2 View

Is nr blast important?

Hello, I have designed a specific primer for my Gene, when I blast this primer, it just finds the mRNA of the gene, but in nr blast, I have found some CDSs that are not relevant to my Gene. Do...

05 February 2021 5,842 3 View

Apart from MOFA what other software is available for analysis of a multi-omic study?

Study bringing together genomics, proteomics etc together

02 February 2021 2,856 2 View

Abaqus - plate is going up under gravity+blast loads?

Hi, I have modeled concrete plate with reinforcement supported on 2 concrete block. between concrete block and the plate I have added regular contact. I have created 2 steps: 1) only gravity load...

30 January 2021 2,772 1 View

Qiime2- No reads passed the filter. How to set the trunc value?

Hi, I am working on some 16S sequencing data, and seems some of them are low quality. I am not sure how to set the trunc value in dada2. qiime dada2 denoise-paired \ --i-demultiplexed-seqs...

30 January 2021 6,213 3 View