Meniscus formation matrigel?

Hey guys, I am currently doing tube formation assay on matrigel coated coverslips, and i wonder if there is any possibility to get rid of the meniscus formation? I have only a small part of the...

09 February 2021 6,979 3 View

How could alanine substitution vs deletion have an impact on a protein?

I performed deletion mutagenesis along the c terminal region of a protein targeting two specific leucine residues, and showed no change compared to wild type, as assayed by dual...

05 February 2018 4,380 2 View

Redundant non-target deletion next to desired mutation site? (PCR site-directed mutagenesis)

20 August 2017 8,046 2 View

Are Gomori buffers prepared in the ratios given or do they require additional titration to obtain the correct pH?

I have been trying to prepare some buffers for pH assays in the range of 1-11. Starting with pH1, I noticed that mixing the solutions as shown in the table does not give the correct pH. Instead,...

24 February 2015 4,553 4 View

How long do you use Versene to detach adherent hepatocyte cell line (for further facs analysis)?

17 February 2014 2,346 2 View

Does anyone know of a validated questionnaire examining social isolation and or homophobia that I could access?

The Liverpool Mental Health Consortium LGBT group are planning on administering a questionnaire to the LGBT Liverpool community

08 January 2014 4,218 2 View

What's the most relevant prostate cancer animal model?

In order to test nutrition or physical activity, I would like some advice on the best prostate cancer animal model: orthotopic or subcutaneous, human or rat or mouse?

04 December 2013 3,492 7 View

BLAST Search Exclusions?

Is there a way of excluding plus/minus strand results in a nucleotide BLAST search?

11 November 2013 2,064 1 View

What is the role of poly-L-ornithine found in a protocol for plate coating with bovine fibronectin?

It seems to be becessary for neuronal cell culture, is it for other cell types?

07 October 2012 3,898 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Can NFL theorem be valid in infinite search space in ML?

NFL theorem is valid for algorithms training in fixed training set. However, the general characteristic of algorithms in expanded or open dataset has not been proved yet. Could you show your...

01 March 2021 1,189 3 View

How much pressure we can reduce at the outlet to avoid Reverse Flow?

I am simulating Heat Exchanger. I have tried and tested all the methods to resolve this issue from the internet like refining mesh, improving skewness and orthogonal quality ,extending outlet to...

01 March 2021 6,985 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View