Dear all,
Does someone know what these black EVs are in my UC preparation from a human cancer cell line (not known to be infected with any virus)?
As you can see in the TEM scan, not only we have cup-shaped EVs of various sizes, but also these black/dark vesicles of non-constant diameter. Cells were checked for mycoplasma and did not look infected by anything as they grew normally and looked great under the microscope.
Could they just be a vesicle subpopulation? Maybe some protein aggregates?
Please let me know your thoughts on the topic.
Best regards,
Julia
I suspect that these are exosomes. Many, if not all, cells produce them.
I have attached a document with a TEM pic of exosomes produced by cultured CD34+ umbilical cord blood hematopietic stem cells (sorry, I had to download it from a ppt file, and could not get it into tif form).
There is an extensive literature about exosomes from the early '10s.
hi,
for further identification I would suggest to do immunolabeling, general exosomal markers and some cell/cancer specific markers.
kind regards
ketil
Dear Julia UC remains the gold standard to assess the purity of EV preparations. Look for ultrasensitive detection of cancer biomarkers by nickel-based isolation of polydisperse EVs. Regards
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