Ayodeji Abijo

10 Questions 20 Answers 0 Followers

Questions related from Ayodeji Abijo

Why are the internal controls (Actin and GAPDH) absent in my extracellular vesicles (EVs) samples?

I isolated EVs from iPSC-derived NSC lines using a commercial kit and characterized the Evs for presence of classical EV positive Markers and detected the expression of CD63, TSG101, and Calnexin...

07 November 2024 4,555 2 View

Why is my Antibody not detecting mature BDNF in Western Blot?

I am trying to detect the expression of BDNF in hippocampal tissues with western blot via the chemiluminescence method; however, I noticed that only pro-BDNF bands were present at approximately 37...

25 October 2023 7,369 0 View

What causes iPSC death after Passaging?

I am working with DF-19 iPSC, I noticed that my cells attach just fine on vitronectin-coated plate on the first day. However, a day after, I noticed that my cells undergo massive death. I have...

05 June 2023 3,082 7 View

Best Transfection Method for Neural Stem Cells?

We aim to express the green fluorescent protein (GFP) in cultured neural stem cells for calcium imaging studies. Which of the transfection methods (electroporation or lipofectamine) is highly...

13 May 2023 1,052 0 View

How do I determine seeding number in cell culture Study?

I am entirely new to cell culture studies and I need clarification on determination of seeding number after cell count with Neubauer hemocytometer. Say I have a cell density of about 4x10^6 and I...

09 March 2023 1,379 2 View

Can Fiji Image J be used for measurement of extent of basement membrane invasion by cancerous cells in DMBA model of tumorigenesis in Wistar rats?

We induced breast tumors in Wistar rats using DBMA (7,12, Dimethyl benzathracene). We aimed to measure the extent of basement membrane (BM) invasion by cancerous cells. The PAS technique was used...

01 May 2021 1,462 0 View

Blood pressure determination in rats

Can anyone please describe the procedure for blood pressure determination in rats 🐀. Also, what type of instrument is used for this?

01 January 1970 3,954 6 View

Assessment of Anxiety

I have come across different ways to measure anxiety in Wistar rats but I get confused sometimes. Using the open field box as a tool, I know that reduced locomotory activity is an indicator of...

01 January 1970 4,474 5 View

Fluorescent Labelling of Extracellular Vesicles With PKH dye

I am currently working on extracellular vesicles (EVs) and I intend to perform uptake/internalization assay using SH-SY5Y neuroblastoma cell line. I experimented on the PKH26 dye which could be...

01 January 1970 9,283 0 View

Extracellular Vesicle (EV) Markers (CD9, CD63 and CD81) Troubleshooting

I isolated extracellular vesicles from iPSCs-derived Neural stem cells via a combination of ultracentrifugation with a commercial kit and characterized the EVs using NTA to determine the size...

01 January 1970 7,167 0 View