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Questions related from Ayodeji Abijo
I isolated EVs from iPSC-derived NSC lines using a commercial kit and characterized the Evs for presence of classical EV positive Markers and detected the expression of CD63, TSG101, and Calnexin...
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I am trying to detect the expression of BDNF in hippocampal tissues with western blot via the chemiluminescence method; however, I noticed that only pro-BDNF bands were present at approximately 37...
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I am working with DF-19 iPSC, I noticed that my cells attach just fine on vitronectin-coated plate on the first day. However, a day after, I noticed that my cells undergo massive death. I have...
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We aim to express the green fluorescent protein (GFP) in cultured neural stem cells for calcium imaging studies. Which of the transfection methods (electroporation or lipofectamine) is highly...
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I am entirely new to cell culture studies and I need clarification on determination of seeding number after cell count with Neubauer hemocytometer. Say I have a cell density of about 4x10^6 and I...
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We induced breast tumors in Wistar rats using DBMA (7,12, Dimethyl benzathracene). We aimed to measure the extent of basement membrane (BM) invasion by cancerous cells. The PAS technique was used...
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Can anyone please describe the procedure for blood pressure determination in rats 🐀. Also, what type of instrument is used for this?
01 January 1970 3,960 6 View
I have come across different ways to measure anxiety in Wistar rats but I get confused sometimes. Using the open field box as a tool, I know that reduced locomotory activity is an indicator of...
01 January 1970 4,483 5 View
I am currently working on extracellular vesicles (EVs) and I intend to perform uptake/internalization assay using SH-SY5Y neuroblastoma cell line. I experimented on the PKH26 dye which could be...
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I isolated extracellular vesicles from iPSCs-derived Neural stem cells via a combination of ultracentrifugation with a commercial kit and characterized the EVs using NTA to determine the size...
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