Best software for Western blot band quantification?

More Kristen Navarro's questions See All

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

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How to get onecolumn document from standard IEEE ACCESS LateX template?

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Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

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01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

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How to remove DCU and HOBT from my reaction?

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28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

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How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

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B-mercaptoethanol (BME) protocol?

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What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

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24 February 2021 8,219 2 View

How to quantitative analize the data of TNBS assay for gelatin?

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How to accurately quantify chitin?

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Will a 10xHis-tag prevent anti-MBP from binding to MBP?

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25 February 2021 8,404 3 View

What's the concentration recommend for recombinant protein use in western blotting?

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What is the best way to manage Western blots?

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23 February 2021 8,609 3 View

Best plasmid could be used as a standard curve for AAV titration?

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How to manage western blot for proteomics data validation?

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Diltuions for a Western blot?

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22 February 2021 9,901 3 View

Can anyone help me how to get the similar bands in Actin?

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22 February 2021 6,937 3 View

I see 2 random bands in my ERK/pERK blot? Has anyone else seen this?

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Goodwin G. Jinesh

ImageJ

Its free

George Víctor Pérez Viltre

Hi Kristen Navarro, you can download ImageJ for free, it is compatible for Mac and Windows laptops.

Nandaraj Taye

Hello I use ImageJ for quantifying western blot bands. Here are some of my video tutorials to download Imagej software for mac and quantify. hope you find it useful

https://m.youtube.com/watch?v=GfNQVDh_uEI

https://m.youtube.com/watch?v=u-u3G7JhIAo

Steven Dodd

Hi Kristen Navarro, you can trial a free copy of our Phoretix 1D image analysis software for your Western Blots here: https://totallab.com/products/phoretix-1d/

It's compatible with all Macs and Windows 11, features user-customisable automation to process lots of blots very quickly and is much more user-friendly and saves a lot more time than using ImageJ.

Best wishes,

Steven