Is there any phosphates sensor (or total phosphorus) for online monitoring of the lakes?

We need to monitor surface waters in terms of nutrients and algae content. We are building up an on-line in-situ monitoring system for early detection of cyanobacterial blooming and toxicity into...

27 February 2020 9,612 7 View

Would you be interested in hosting a Fulbright Visiting Scholar?

I would like to apply for a Fulbright Visiting Scholarship, but I need a colleague who would like to host me in a U.S. University. My research interests include Special and Inclusive Education,...

08 October 2019 3,089 3 View

Best method for dna extraction from honey ?

hello, what is the best method for extracting bacterial dna from honey? thank youu

19 February 2019 131 6 View

Does anyone have any experience with RET immunohistochemistry?

Does anyone have any experience with RET immunohistochemistry? I am interested in staining nerves in skin. Thank you

09 November 2016 7,648 2 View

Is there a way to increase the sensitivity in the "do it yourself/ready-set-go" kits?

19 October 2016 8,696 3 View

What demethylating reagent do you suggest?

I would like to demethylate a substrate which carries one aryl methoxy group and heterocycle moieties. I tried some methods by using HBr and BBr3 but they didn't give any demethylated product. My...

03 August 2016 4,711 19 View

Is anybody working on architectural spaces and/or urban places or neighbourhood projects aiming at well-being of people with dementia condition?

02 August 2016 7,382 8 View

What is the best survey/questionnaire to assess children's perceptions on weapons?

We are conducting a study on primary school children's perceptions of what constitutes a weapon in Greece.

02 July 2015 1,407 2 View

Can I use boron tribromide for the demethylation of a methoxy group on a compound which carries also a primary arylamine?

Do I have to protect the amine?

28 July 2014 3,785 3 View

Imaging extracellular membrane proteins

I want to visualize a protein which will reach the plasma membrane and localize on the extracellular space while performing live cell imaging. Is there a way to have a tag, which will only become...

03 November 2013 582 9 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Suggest me some trending topics for phd research in text mining/AI/machine learning?

My master's research is in info retrieval and text mining. I would be grateful if you could help me to select a good topic for my phd research proposal.

24 February 2021 393 14 View

Does qualitative research using closed and open ended survey and semi structured interviews qualify as triangulation?

I am using qualitative research to look at employee motivation. I will use a closed and open ended survey (for biodata information and general views to motivation) and semi structured interviews...

18 February 2021 436 11 View

How to find standard axial compressor blade data files ?

I'm looking for standard axial compressor blade row data files for CFX simulation , so could somebody tell me where can I find them ? Like rotor37 , PBS rotor , and something else about them...

13 February 2021 7,745 3 View

How we can get maximum benefit from Big Data utilization in our life?

Big data is a field that treats ways to analyze, systematically extract information from, or otherwise deal with data sets that are too large or complex to be dealt with by traditional...

13 February 2021 7,347 6 View

How to model in HFSS or FEKO Free Space Material Characterization?

I am having trouble setting up this problem in HFSS or FEKO. In HFSS, I've tried both FEM and FEBI-IE solvers. With FEKO, I've tried it's MoM solver and default settings. I am would like model...

09 February 2021 375 2 View

How can I do antigen retrieval for proliferative markers (MCM2, Ki67 etc) While preserving a sensitive endogenous reporter?

I am trying to do antigen retrieval on mouse brain sections to stain for proliferative markers like MCM2, Ki67, PCNA etc on tissue that has mCherry conjugated to a transcription factor of interest...

18 January 2021 1,023 3 View

Can anyone walk me through IHC/IF Staining of Cryosectioned Brain Tissues?

I'm looking to do some IHC/IF staining of X. laevis brains that have been frozen in OCT media for cryosectioning. I understand how to do the cryosectioning, but I am hoping for help/protocols that...

14 January 2021 9,752 2 View

Any recommendation on SaCas9 antibody?

Hi, does anyone know a good SaCas9 antibody for IHC or immunofluorescence on mouse brain slides? We gave a try with the Epigenetek monoclonal antibody (A-9001) without antigen retrieval (1:500)...

29 December 2020 4,368 5 View

Best method to detect cell death in the brain of adult zebrafish on sections?

Hello all, I am trying to detect cell death in the brain of adult zebrafish on sections. I performed immunohistochemistry on brain sections using the Purified Rabbit Anti- Active Caspase-3...

14 December 2020 9,940 3 View