Prakash Chandra

6 Questions 86 Answers 0 Followers

Questions related from Prakash Chandra

Regarding to presentation of pairwise comparison of data?

While comparing data pairwise we see that researchers represent data table and use the phrase: "Values (MEAN±S.E.M.) in the same column with different superscript differ significantly...

23 January 2021 8,819 1 View

Number of competent In vitro produced embryos lost per year ?

dear friends , Is any authenticated data or research is available that shows approximate or exact number of in vitro produced competent embryos lost every year due to unavailability of...

21 October 2020 1,489 3 View

On Culture COCs show Fibroblast like morphology could they be considered and can be used as fibroblasts?

When we culture COCs (cumulus Oocyte complexes) for IVM (in-vitro Maturation) Cumulus cells show morphology similar to fibroblast cells. have few queries: 1. Could we consider them...

09 October 2018 9,399 3 View

Regarding to expression of specific gene ?

I want to know expression level of a certain genes I do have primers . I have few questions: 1. is it necessary for me to quantify RNA? if it is only 1 sample? 2. Is it necessary that i should...

17 April 2017 838 3 View

Can anoyone help with a problem in the 5'RACE?

I am working on molecular cloning of a gene we have already synthesized the 3'end of CDS now we want to clone 5' end and for the same I am following 5'RACE strategy. In final step with PCR anchor...

16 May 2014 1,112 8 View

We used to take O.D at 600 nm for s culture. Could somebody tell me why we use mostly 600 nm and why not more or less?

I know if we use UV range it will harm the cells.

15 April 2014 1,320 7 View