Prakash Chandra

13 Questions 136 Answers 0 Followers

Questions related from Prakash Chandra

Regarding to presentation of pairwise comparison of data?

While comparing data pairwise we see that researchers represent data table and use the phrase: "Values (MEAN±S.E.M.) in the same column with different superscript differ significantly...

23 January 2021 8,925 1 View

Number of competent In vitro produced embryos lost per year ?

dear friends , Is any authenticated data or research is available that shows approximate or exact number of in vitro produced competent embryos lost every year due to unavailability of...

21 October 2020 1,550 3 View

On Culture COCs show Fibroblast like morphology could they be considered and can be used as fibroblasts?

When we culture COCs (cumulus Oocyte complexes) for IVM (in-vitro Maturation) Cumulus cells show morphology similar to fibroblast cells. have few queries: 1. Could we consider them...

09 October 2018 9,461 3 View

Regarding to expression of specific gene ?

I want to know expression level of a certain genes I do have primers . I have few questions: 1. is it necessary for me to quantify RNA? if it is only 1 sample? 2. Is it necessary that i should...

17 April 2017 892 3 View

Dear researchers is there any online tool that could tell about the constitutive gene of particular tissue sample in particular animal ?

i need few questions to be solved: 1. I need to know about constitutive genes of bovine Cumulus cells although found 4 genes Viz. 18srRNA ,RPL19,PPIB,GAPDH. want best of 2 from 4 genes. any...

17 February 2017 8,451 1 View

i need help regarding cryopreservation of female gamete in bovines?

I am trying to cryopreserve immature bovine oocytes in .25 ml french straws by vitrification but i am facing problems: 1. I have used almost all vitrification media composition and protocols...

08 December 2016 385 19 View

Survival time of bovine oocyte after maturation in a media?

I am just curious to know what is the maximum period an unfertilized mature oocyte could survive in a media in appropriate culture conditions. thank you

22 August 2016 1,377 11 View

I am surprised why our oocyte (cumulus oocyte complex) sample sometimes forms a jelly like structure ?

we work in IVF of bovine oocytes . 1. After aspiration when we collect COCs (cumulus oocyte Complex) to washing media it sometimes forms a jelly like structure . (I cannot use it further) 2....

14 July 2016 2,370 11 View

We are going for In vitro embryo production of Bovine (Bos indicus )but we are nor reaching till Balastocyst stage ?

so far I have tried co-culture with oviductal cells in K-RVCl-50 media ( culture conditions are 38.5 ° C and 5% CO2 ) still we are reaching till morulla stage after that the embryos are...

13 April 2016 727 12 View

How can I improve live sperm concentration after 2nd washing with FBO (washing media) during IVF? Specifically in caprine(goat) species sperm?

I want to go for IVF but after washing with washing media I am getting very low concentration of live sperm . dear colleagues any suggestions ? thanks In advance

11 September 2015 7,019 5 View

Can anoyone help with a problem in the 5'RACE?

I am working on molecular cloning of a gene we have already synthesized the 3'end of CDS now we want to clone 5' end and for the same I am following 5'RACE strategy. In final step with PCR anchor...

16 May 2014 1,177 8 View

We used to take O.D at 600 nm for s culture. Could somebody tell me why we use mostly 600 nm and why not more or less?

I know if we use UV range it will harm the cells.

15 April 2014 1,395 7 View

Effective use of In-vitro Produced Embryos

Welcome to all, This discussion is based on the effective use of In-vitro produced embryos . what i have seen is that after prolonged culture the hatched blastocyst they start to swell and finally...

01 January 1970 721 1 View