We're currently looking forward to star doing silencing experiments on cultured rat cells (Mesenchymal stem cells). I came across two very different methods, one by transfection of plasmids (pLKO.1 for example), and the other one is using directly the siRNA molecules bypassing the bacteria (Stealth™ RNAi). I really didn't look anything about viral vector because i don't have any experience with virus culture.
Have anyone try one or both ? Which one would be better and/or cheaper?
Thank you so much.
Hi Agustina,
I think the RNAi mediated silencing through lentiviral transfection is most recommended for stable integration and long term knock down of your targeted gene. I have read Very recent works attached in next link and get more information.
https://www.nature.com/articles/ncomms6375
1 st is the most cheap
Hi
Both techniques are good but siRNA cheaper than other techniques
kindly see the links
Good luck
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3226283/
http://onlinelibrary.wiley.com/doi/10.1002/jbmr.2816/full
These links may help you
https://www.researchgate.net/post/Which_is_better_for_studying_siRNA_miRNA_silencing_a_growing_cell_layer_or_a_confluent_monolayer
https://www.thermofisher.com/us/en/home/references/ambion-tech-support/rnai-sirna/general-articles/top-ten-ways-to-optimize-sirna-delivery-in-cultured-cells.html
http://www.sigmaaldrich.com/life-science/functional-genomics-and-rnai/shrna/learning-center/mission-faqs/set-up-experiments.html
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1370783/
Article Design and delivery of antisense oligonucleotides to block m...
Chapter Methods for High-Throughput RNAi Screening in Drosophila Cells
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