I did the biosynthesis of silver nanoparticles using plant leaf extracts. After the extract was reacted with AgNO3 with a volume ratio of 5: 1 (extract: AgNO3 1mM), the color of the solution after 24 hours became increasingly dark brown and continued to approach black for up to 96 hours. Initially the color of the solution is dark brown.
The color change, as far as I know, is an indicator of the formation of silver nanoparticles, but peaks do not form at 400-500 nm wavelengths during 96 hours of reaction on the UV-Vis absorbance spectrum. Is there a possibility of phytochemicals in leaf extracts that inhibit the formation of silver nanoparticles or other reasons?
Please have a look at this useful RG link.
Article Green synthesis of silver nanoparticles mediated by traditio...
Please have a look at this useful RG link.
Article Green synthesis of silver nanoparticles mediated by traditio...
may some of antiioxidants as flavonoids may bind to silver. also may dietary fiber
My explanation is: silver nanoparticles were formed when the color changed to brown (did it become red before turning brown?), and here you should have separated those nanoparticles from the reaction mixture and scanned it with a UV-Vis spec.; I think the appearance of black color could've resulted from formation of silver oxide, thus no peaks would appear between 400-500 nm
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