Dear researchers,
May I know is that necessary to add NaCl into the Bradford reagent while doing Bradford assay ?
Thank you.
The main NaCl benefits is its taste. Commonly, we do not like unsalted food.
Concerning Bradford assay . It takes long time and much efforts to develop some assay. Some experimental findings often does not have an explanation. I don't know the answer for your question but my advise is "strictly follow the protocol of an assay." Minor modifications in the assay protocol may result in a catastrophic failure. I did not say that you don't need to understand the basic chemistry behind the assay.
Usually we can't add NaCl to Bradford reagent, you can use in buffer or blank
It is not necessary to add NaCl. If your sample has a high concentration of NaCl or any other constituent, then you should make sure that the same amount is present in all samples and in the standards.
I'm not expert in this area, just curious.
From my point of view, this is a typical trivial question, just google to find the answer. None of recommended receipt contains NaCl. Therefore, I have one more question:"
"May I know is that necessary to add KCl into the Bradford reagent while doing Bradford assay ?" I can ask a couple hundred more similar questions.
Der Jennifer Leong
NaCl have many benefits it absorbs and transports nutrients, maintains B.P, transmits nerve signals.
Its not clear which protocols you are using . I think its not necessary to use NaCl from my experiences. . Of course on the basis of your sample you have to modify any tzpes of established protocols.
I guess you asked this question for the reason that your protein sample is in a saline conatining buffer. Just remember to add equal volume of the buffer to your blank.
All the best!
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