Dear all,

I want to use  EBSP for demographic analyses of several bee species using COI sequence data and thus have several questions:

1.How much sequence variation do you roughly need to make reliable inference (in general my data show rather low variation with an average  nucleotide diversity pi = 0.003)?

2.What is the minimum number of sequences necessary  for EBSP analyses (the lowest I have is 10 per species)?

3. Although I already used 50 Mio. MCMC generations and a sampling frequency of 1,000 I get very low (