If you know protein sequences, you can try programs available via links below.

https://ecpred.kansil.org/

https://enzymedetector.tu-bs.de/

https://loschmidt.chemi.muni.cz/enzymeminer/

May be they will help you.

Piotr Minkiewicz , thank you very much, will definitely try them :)

Okay, let me try to provide some guidance on your approach to finding an enzyme in the stingless bee proteome.

Given that your query sequences are mainly from microorganisms like yeast and bacteria, using BLASTN (nucleotide-nucleotide search) against the bee genome may not be the most effective approach. This is because the gene sequences encoding the enzymes you're interested in may have diverged significantly between microbes and bees.

Instead, I would recommend trying a BLASTP (protein-protein search) approach against the stingless bee proteome obtained from the UniProt database. This is likely to be more successful, as you'll be directly comparing the protein sequences, which are more conserved across species than the underlying nucleotide sequences.

Here are the steps I would suggest:

1. Identify the enzyme or enzymes you're interested in from the microorganism sources. Obtain the protein sequence(s) for these enzymes.

2. Use the BLASTP tool on the UniProt website to search the stingless bee proteome with your query enzyme sequence(s). Make sure to select the appropriate database (stingless bee proteome) and adjust any parameters as needed.

3. Examine the BLASTP results carefully. Look for hits that have a high degree of sequence similarity and coverage to your query enzyme. These are more likely to be the enzymes you're seeking in the stingless bee.

4. If the BLASTP results are not conclusive, you could also try using more sensitive search tools like HMMer or PSI-BLAST, which can sometimes detect more distant homologs.

5. Additionally, you could explore the literature for any published information on the enzymes of interest in stingless bees or other closely related bee species. This may provide valuable clues or direct you to specific protein sequences to search.

The key is to use protein-level comparisons, as this is more likely to reveal the enzymes you're seeking, even if the underlying gene sequences have diverged significantly. Let me know if you have any other questions!