I am looking to optimize the concentration of BAM15 (seahorse agilent) on BV-2 microglial cells. Any suggestion?
dear all, I try to induce neuroinflammation in HMC3 cells using LPS for 24h. The concentration used was 500 ng/ml. But unfortunately no change on TNFa was obtained in LPS versus Control...
29 May 2024 4,007 1 View
I would like to know the meaning of the intensity which appears as y values in the zeta potential distribution graphs provided by Malvern zetasizer instruments. Could you please explain me what is...
22 May 2024 6,855 2 View
I have a chlorophyll corrected Pmax measurement with the unit: µg C (µg Chl)–1 h–1, which I need to convert to µg O2 (µg Chl)–1 h–1, as it is needed for a DO model. I haven't been able to find a...
27 December 2021 4,526 1 View
Hello. I am looking for a good choroidal endothelial cell line to 1- knockdown 2 - perform proliferation and migration assay. I would prefer a murine cell line as I will also incubated these...
17 November 2021 9,622 2 View
I've found online tools to predict protein cleavage sites within unknown proteins, but I'd like to know whether it's possible to do the opposite: given the sequence of an unknown protease, is...
05 August 2021 8,812 3 View
Hello everyone, I am just starting my literature review about the subject and I cannot find an answer to that question. From all I know, no study seems to take the enzyme activity into account...
31 July 2021 5,432 2 View
Hi! Anyone knows of a software that can generate band patterns from whole genomes and custom primers? I have seen it for digestion patterns, but could not find one for PCR. My primers have some...
12 July 2021 7,240 2 View
I'm looking for a software able to process HPLC-UV chromatograms (espacially baseline correction and peak alignement) in order to integrate the data to statistical analysis.
24 May 2021 1,914 10 View
Does anyone know how to access to the software "Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Version 2004A) " ? Otherwise, which other software...
29 October 2020 1,436 2 View
Hi people, My immunofluorescence staining worked well for a long time, but when staining the last 3 plates, I expierence a fluorescent ring in the well (see the picture attached). In some wells...
24 June 2020 3,457 0 View
AGILENT HPLC 1100 SERIES operation assistance needed.
25 July 2024 10,045 2 View
I need to use a photometer, so i have Cary Agilent and i would like to know what kind of cuvettes and its dimensions and volume i have to order. It's about RNA and DNA samples. Which are you...
11 June 2024 712 1 View
Hello fellow researchers, Why don't we see two rRNA peaks as is standard? Our sample was a total RNA prep from A549 cell line. We ran our samples on a pico chip for 2100 Agilent bioanalyzer...
28 May 2024 7,666 0 View
We are an R&D laboratory focused on anionic, cationic, and zwitterionic surfactants and usually tackle surfactant synthesis and analysis of products from customers. In particular, we deal with...
29 April 2024 8,927 1 View
I am wondering what folks have tried for alternative methods of detecting hepatitis B core antigen/capsid. Dako used to make an antibody that was very effective in this and widely used in the...
28 April 2024 3,860 1 View
I am using the new Agilent 990 Micro GC and I am trying to convert my data to excel for all channels. Any idea how to easily do that ?
14 April 2024 8,999 2 View
I would like to measure the oxygen consumption rate directly from tissue in the seahorse XFe96 analyzer with the Seahorse XFe96 Spheroid FluxPak (Part Number:102905-100) but i need a specific glue...
02 April 2024 8,718 1 View
I run an Agilent Technologies Graphite Furnace and a recurring problem when analyzing Lead is that the continuous calibration verification, (CCV), QC slowly drifts lower from the target (20ug/L)....
27 March 2024 3,609 0 View
Lately, I’ve been encountering issues with the longevity of my Liquid Chromatography tandem Mass Spectrometry (LC-MS/MS) columns. Specifically, I use the ZORBAX Eclipse Plus C18 RRHD (Rapid...
26 March 2024 5,129 6 View
I encountered a ZERO TOTAL READ TIME error while working with the ICP device, and I did all the things mentioned in the device's HELP, but the problem still exists, can anyone help me?
10 March 2024 4,560 1 View