What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

RNA extraction from exocrine pancreas?

Hi everyone ! Does anyone have any experience in extracting RNA from exocrine pancreas (which is very high in RNAse)? The pancreas is frozen tissue. I wonder if RNAse is also high in the nucleus,...

31 January 2021 390 2 View

How i can make a ROC curve by using different accuracy values in python ?

I am working on python and I want to show different accuracy values in the ROC curve which will show the different values in curves.

05 March 2020 570 3 View

Can we use DMSO as solvent in nanoprecipitation method?

I am working on synthesis of drug loaded microspheres by using resistant starch as a coating material, my polymer is only soluble in DMSO, and it is nonvolatile in nature how could I possibly...

04 March 2019 7,422 2 View

Is it okay to leave a post-fixed tissue sample of 3-4 mm thickness in 70% ethanol overnight?

We are working on histology of some glands using the paraffin method but since the process is too long and we do not have an automatic processing machine, so we do not have any option other than...

06 February 2019 1,366 1 View

Do you use online educational platforms and which one do you prefer?

There are various online educational platforms, so which one do you use and recommend that teachers use either as a part of their regular course or when teaching an online course?

22 February 2018 736 18 View

How we can calculate the Q-factor for an Optical M-QAM-OFDM signal in DDO-OFDM or CO-OFDM ?

14 August 2017 201 3 View

Problem with polyethylene succinte film formation?

i am having trouble with polyethylene succinate film formation, i am using 0.1% polyethylene succinate concentration with 10% dichloro methane as a solvent. the film is not hard enough to be used...

12 December 2016 5,037 1 View

Concentration of triton x 100 in THP method for DNA extraction?

Hi. Recently I start working on THP protocol for DNA extraction to optimize the yield of circulating cell-free DNA from plasma. I am mixing 5 ul of Triton X 100 in 500 ul of plasma sample but I...

17 October 2016 6,216 3 View

How to extract keratin from human hair?

Keratin is a protein and essential component of hair, what is the right and easy way to extract keratin protein from human hair , what the solvents used to break the sulfide bonds between the...

27 August 2016 8,173 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

Does anyone know, can i do cryosectioning of whole aorta after oil red O staining and use the cryosection parts for further analysis?

I would like to quantify the lesions on aorta. I want to take image of atherosclerotic plaques formation in aorta by using O red Oil Solution. After the staining, what will be the storage...

16 February 2021 5,876 2 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

What is the optimal method to prepare a controlled porosity osmotic pump (CPOP) tablet for scanning electron microscopy?

In order to assess the shape and size of pores formed by dissolving the pore former in the semipermeable membrane structure, SEM images are needed. Cellulose acetate was used in the coating...

15 February 2021 6,135 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View