Hi! everyone. I need someone help to understand wb results. I got two band of different sizes, actually it is the sum of my actual band. Is there any possibility that my protein has been split or break into two parts after induction. If my protein has been split then, what are the possibilities behind it.
Well, there are several options. It would help to know a little more about your overall experiment. Is the antibody to your protein or to a tag? Has the antibody been tested before and if so what did the results look like?
Assuming this is a protein-specific antibody, it is possible that your antibody recognizes more than just your one protein. If you have a knockout of your target protein in your organism that would help figure out if there is non-target binding.
It is also possible that your protein has splice variants, or is being cleaved, or has processing steps. You would only be seeing one part of any cleavage products as the epitope would be absent on one of the fragments.
Hope this helps!
I can only add maybe: for some proteins it's very common to show double bands, depends greatly on your protein and/or method/treatment. Maybe your protein undergoes some Post translational modification!?
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