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Questions related from Guanqun L. Liu
Dear all, I've spent a long time struggling with DAPI staining of transfected 293T cells on poly-L-lysine coated chamber slides. I'm using TransIT-LT1 (Mirus Bio) as the transfection reagent and...
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After cutting the band out of the 8M Urea gel, what should I do to elute the RNA? Which elution buffer should I use and how to clean up the salt? Is phenol-chloroform extraction a necessary step...
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(see attached diagram) I am doing a two-step qPCR. I incorporated different point mutations at the 3' end of my target RNA and am planning to measure the difference in RNA expression level using...
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