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Questions related from Guanqun L. Liu
Dear all, I've spent a long time struggling with DAPI staining of transfected 293T cells on poly-L-lysine coated chamber slides. I'm using TransIT-LT1 (Mirus Bio) as the transfection reagent and...
26 August 2016 3,652 12 View
Dear all, I'm checking the cellular localization of some proteins in 239T cells but they adhere poorly on chamber slide and come off easily during staining procedures. Does anyone have a better...
07 December 2015 9,966 7 View
Hi all, Does anyone have done selection of monoclones from cells that are grown in suspension? In our case, we need to create stable THP-1 cell lines using puromycin. One specific question is that...
16 September 2015 2,624 3 View
After cutting the band out of the 8M Urea gel, what should I do to elute the RNA? Which elution buffer should I use and how to clean up the salt? Is phenol-chloroform extraction a necessary step...
12 May 2015 2,012 6 View
I'm trying to propagate a H1N1 strain in DMEM plus 0.2% BSA and 1 ug/ml TPCK-trypsin but mock treated cell (uninfected) died after 24 h. I repeated twice and the same phenomenon happened. This...
17 April 2015 454 5 View
My immunoblotting failed to detect a protein of interest and I'm using the M-PER™ Mammalian Protein Extraction Reagent, which could efficiently extract both cytoplasmic and nuclear proteins...
15 April 2015 861 5 View
(see attached diagram) I am doing a two-step qPCR. I incorporated different point mutations at the 3' end of my target RNA and am planning to measure the difference in RNA expression level using...
16 March 2014 4,991 5 View
