I am looking for the details of BSA attachment to PDMS microfluidic channels.
1) I have a PDMS microfluidic channel and I flow FITC-BSA (in PBS buffer).
2) I have an APTES coated PDMS microfluidic channel. I flow FITC-BSA (in PBS buffer).
I want to know whether BSA will attach to the the PDMS channels or not ?
3) BSA is used extensively to decrease non-specific adsorptions. If someone is interested in quantifying BSA protein itself, what exactly is the mechanism. Especially in microfluidic domain where one has a fictionalized or non-fictionalized channel.
Note: I am interested in simple affinity based protocols (like avidin-biotin), specific no to usage of antibodies.
I guess the non-specific BSA adsorption could due to electric charge? A quick test would be using buffers with different ionic strength to see if there is a difference in adsorption?
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