How can I attach fluorescence tagged Avidin (e.g., FITC Avidin) to APTES coated microchannels without using biotin ?

I am looking to attach avidin to the surface of a PDMS microfluidic channel coated with APTES. I have attached simple avidin molecule using EDC/NHS conjugation protocol (without using biotin or...

11 December 2017 3,778 3 View

Attachment of BSA (or FITC BSA) to PDMS microfluidic channels ?

I am looking for the details of BSA attachment to PDMS microfluidic channels. 1) I have a PDMS microfluidic channel and I flow FITC-BSA (in PBS buffer). 2) I have an APTES coated PDMS...

11 December 2017 1,310 1 View

How to quantify capture efficiency of antigens in a microfluidic immunoassay ?

How can one quantify capture efficiency of antigens in a microfluidic immunoassay chip ? For example, if we have a anti-IgG coated microfluidic chip and we want to know the capture efficiency of...

09 October 2017 1,298 7 View

I need to detect/quantify concentration of proteins attached to antibodies ?

I am capturing proteins using cleavable linkers. The released (eluted sample after pre-concentration step) sample have the antibody attached to the captured proteins. I can recapture them using...

09 October 2017 1,902 3 View

How to conjugate "PSA anti-bodies" with "NHS - esters" ?

I am looking for conjugation mechanism to conjugate a "photo-cleavable (PC) Biotin - NHS ester" to "PSA anti-bodies". I need the Biotin part for the Avidin/Biotin bonding on the surface and...

05 June 2016 2,175 2 View

Which is the best "Fluorescent tagged Biotin" one should use for fluorescence measurement in order to determine Avidin Coating on Silicon Sample ?

I am coating silicon sample with Avidin and want to characterize the surface coating. I am looking for fluorescent measurements using "fluorescent tagged biotin" binding to Avidin surface. Please...

02 March 2016 208 0 View

How to conjugate "Fluorescein Biotin" to avidin coated silicon surface ?

I have avidin coated silicon surface. I want to conjugate "Fluorescein Biotin" to that. I have obtained "Fluorescein Biotin" from http://www.scbt.com/datasheet-214340-Fluorescein-Biotin.html I am...

01 February 2016 8,249 1 View

Can I conjugate Avidin on APTES coated surface without using Biotin?

I am looking to attach "Avidin" to APTES coated silicon surface without using biotin bonding. What I have in mind is to use "EDC or EDAC + NHS + avidin solution" and immerse the APTES coated...

31 December 2015 754 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What kind of intervention will be relevant to increase motivation among young sport trainees?

Any suggestion on what book to read is also welcome.

03 March 2021 1,646 4 View