How can I attach fluorescence tagged Avidin (e.g., FITC Avidin) to APTES coated microchannels without using biotin ?

I am looking to attach avidin to the surface of a PDMS microfluidic channel coated with APTES. I have attached simple avidin molecule using EDC/NHS conjugation protocol (without using biotin or...

11 December 2017 3,778 3 View

Attachment of BSA (or FITC BSA) to PDMS microfluidic channels ?

I am looking for the details of BSA attachment to PDMS microfluidic channels. 1) I have a PDMS microfluidic channel and I flow FITC-BSA (in PBS buffer). 2) I have an APTES coated PDMS...

11 December 2017 1,310 1 View

How to quantify capture efficiency of antigens in a microfluidic immunoassay ?

How can one quantify capture efficiency of antigens in a microfluidic immunoassay chip ? For example, if we have a anti-IgG coated microfluidic chip and we want to know the capture efficiency of...

09 October 2017 1,298 7 View

I need to detect/quantify concentration of proteins attached to antibodies ?

I am capturing proteins using cleavable linkers. The released (eluted sample after pre-concentration step) sample have the antibody attached to the captured proteins. I can recapture them using...

09 October 2017 1,902 3 View

Which is the best "Fluorescent tagged Biotin" one should use for fluorescence measurement in order to determine Avidin Coating on Silicon Sample ?

I am coating silicon sample with Avidin and want to characterize the surface coating. I am looking for fluorescent measurements using "fluorescent tagged biotin" binding to Avidin surface. Please...

02 March 2016 208 0 View

How to conjugate "Fluorescein Biotin" to avidin coated silicon surface ?

I have avidin coated silicon surface. I want to conjugate "Fluorescein Biotin" to that. I have obtained "Fluorescein Biotin" from http://www.scbt.com/datasheet-214340-Fluorescein-Biotin.html I am...

01 February 2016 8,249 1 View

Can I conjugate Avidin on APTES coated surface without using Biotin?

I am looking to attach "Avidin" to APTES coated silicon surface without using biotin bonding. What I have in mind is to use "EDC or EDAC + NHS + avidin solution" and immerse the APTES coated...

31 December 2015 754 5 View

Looking for a soft copy of a book titled "Electric Power Generation, Transmission, and Distribution" by S.N.Singh"

I have a hard form of the book with me. I am looking to have a .pdf version of it. Please share any link where I can have it. Thank you

01 January 1970 9,667 9 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View