Ahmad Usman

9 Questions 23 Answers 0 Followers

Questions related from Ahmad Usman

How can I attach fluorescence tagged Avidin (e.g., FITC Avidin) to APTES coated microchannels without using biotin ?

I am looking to attach avidin to the surface of a PDMS microfluidic channel coated with APTES. I have attached simple avidin molecule using EDC/NHS conjugation protocol (without using biotin or...

12 December 2017 3,831 3 View

Attachment of BSA (or FITC BSA) to PDMS microfluidic channels ?

I am looking for the details of BSA attachment to PDMS microfluidic channels. 1) I have a PDMS microfluidic channel and I flow FITC-BSA (in PBS buffer). 2) I have an APTES coated PDMS...

12 December 2017 1,360 1 View

I need to detect/quantify concentration of proteins attached to antibodies ?

I am capturing proteins using cleavable linkers. The released (eluted sample after pre-concentration step) sample have the antibody attached to the captured proteins. I can recapture them using...

10 October 2017 1,953 3 View

How to quantify capture efficiency of antigens in a microfluidic immunoassay ?

How can one quantify capture efficiency of antigens in a microfluidic immunoassay chip ? For example, if we have a anti-IgG coated microfluidic chip and we want to know the capture efficiency of...

10 October 2017 1,343 7 View

How to conjugate "PSA anti-bodies" with "NHS - esters" ?

I am looking for conjugation mechanism to conjugate a "photo-cleavable (PC) Biotin - NHS ester" to "PSA anti-bodies". I need the Biotin part for the Avidin/Biotin bonding on the surface and...

06 June 2016 2,228 2 View

Which is the best "Fluorescent tagged Biotin" one should use for fluorescence measurement in order to determine Avidin Coating on Silicon Sample ?

I am coating silicon sample with Avidin and want to characterize the surface coating. I am looking for fluorescent measurements using "fluorescent tagged biotin" binding to Avidin surface. Please...

03 March 2016 247 0 View

How to conjugate "Fluorescein Biotin" to avidin coated silicon surface ?

I have avidin coated silicon surface. I want to conjugate "Fluorescein Biotin" to that. I have obtained "Fluorescein Biotin" from http://www.scbt.com/datasheet-214340-Fluorescein-Biotin.html I am...

02 February 2016 8,299 1 View

Can I conjugate Avidin on APTES coated surface without using Biotin?

I am looking to attach "Avidin" to APTES coated silicon surface without using biotin bonding. What I have in mind is to use "EDC or EDAC + NHS + avidin solution" and immerse the APTES coated...

01 January 2016 806 5 View

Looking for a soft copy of a book titled "Electric Power Generation, Transmission, and Distribution" by S.N.Singh"

I have a hard form of the book with me. I am looking to have a .pdf version of it. Please share any link where I can have it. Thank you

01 January 1970 9,718 9 View