I am working with nuero2a cells and treating them with a protein that has been prepared in DMSO (3)%. I am wondering at what percentage might this DMSO kill my nuero2a cells.
I can't say for Neuro2a specifically, but DMSO is usually tolerated with little or no observable effects at 0.1% final concentration (v/v). At 1% or higher, effects on cell behavior have been reported.
Is your protein/peptide in 100% DMSO, and you are diluting it 33-fold for a final concentration of 3% DMSO, or is your protein in 97% aqueous solution, 3% DMSO? If the latter, then try to make sure your protein stock is at least 30x more concentrated than the working concentration. If the former, you may need to try to change solvents or concentrate your protein before use.
Not sure avout Neuro2a, but for L929 cells, the IC50 value of DMSO was around 2%
For SH-SY5Y is recommended to use 0,1% at final concentration.
I am not sure about Neuro2a, but for HL-60 , PC-3 and HepG2 cells, the toxicity of DMSO can be found around 2% of DMSO
Hi, can you please attach a published article that discusses the toxic DMSO levels, esp for SY5Y cells?
Thanks
Just did an experiment, where 1.6% DMSO turned out to be pretty toxic for colon cancer cells. Because we did not titrate lower, it basically scrambled the experiment.
Again learned the hard way to never forget to do the proper controls, even if you think about saving yourself the work of these extra wells.
Only thing to say, Antonio is absolutely right.
I did an experiment where I used 2% DMSO in SH-SY5Y cells and I saw almost 50% cell death. I did not do the calculations right that's why I ended up having 2% DMSO per well.
I repeated with correct calculations and reduced the final concentration of DMSO to 0.01%. No cell death in Control.
Evaluation of the Cytotoxicity Effect of Dimethyl Sulfoxide (DMSO) on
Caco2/TC7 Colon Tumor Cell Cultures
Georges DA VIOLANTE,a,b Naima ZERROUK,b Isabelle RICHARD,a Gérard PROVOT,a
Jean Claude CHAUMEIL,b and Philippe ARNAUD*,c
Hi Ross,
I do work with Neuro2A cells on regular basis and I felt that these cells are pretty sensitive and it's better that you should keep your DMSO conc. [finally in one well] less than 0.1%. Please see my publications
http://pubs.rsc.org/en/content/articlepdf/2012/ob/c2ob26107c
http://www.nature.com/articles/srep14134
Hope it helps in planning your next experiment.
All the best
Sumana
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