JT Ross

5 Questions 3 Answers 0 Followers

Questions related from JT Ross

What are the best conditions for getting separation between different conformations of HIV-1 RNA, using gel electrophoresis?

Currently I am trying different concentrations of HIV RNA, 1um, 4um, 6um, and 10um. I'm wondering if anyone has tried this perhaps in another RNA virus model and had success; by changing pH or...

10 October 2014 2,029 4 View

Does utilizing EDTA to stop transcription influence the degradation of RNA?

I currently stop transcription reactions of HIV RNA with EDTA. I am wondering if this could contribute to any unwanted degradative effects as I am seeking the highest yield possible of RNA.

09 September 2014 2,715 3 View

At what percentage is DMSO considered non toxic to cells?

I am working with nuero2a cells and treating them with a protein that has been prepared in DMSO (3)%. I am wondering at what percentage might this DMSO kill my nuero2a cells.

05 May 2013 5,060 10 View

Any suggestions for a review on the foundations of electron microscopy-scanning EM, Transmission EM, and STEM?

Looking for any review on this topic--does not have to be recent.

04 April 2013 866 1 View

What are good options for solvents of Abeta-42 to be used in a N2a cytotoxicity assay (other than PBS, HAMS F-12, and DMEM ph 6.5)?

I cannot get my AB-42 oligomers to kill N2a cells plated at around 40% confluence in serum free media during treatment with oligomers. We originally dissolved the AB-42 film in HAMS F12, but we...

04 April 2013 2,489 7 View