Hi Nadhirah,

These could perfectly be dimeric configurations of your product. Notice that MW is almost 2x that of your product. In any case these are NOT unspecific products.

Cheers

Hi Nadhirah, 

Which species of the the RNA from? Some species have more / less number of ribosomal peaks. 

Anna

Dear Anna,

I extract total RNA from human cell lines..

Dear Nad 

to obtain the 2 canonical bands you mention you need to denature your samples. If you are not already doing this secondary structure can occur complicating the picture. Thus: 

I think third band is of RNA (28S) because  rather than running on denaturing agarose gel you have run the RNA sample on simple agarose and due to secondary in structures/different oonfirmation in RNA ,more than two band appear. hypothetically if you don't use denaturing agarose gel, more than two band of RNA can appear.

Best wishes

Reference denature RNA gel picture (from ThermoFisher Scientific): https://www.thermofisher.com/us/en/home/references/protocols/nucleic-acid-purification-and-analysis/rna-protocol/agarose-gel-electrophoresis-of-rna.html

Dear Laurence and Naveed,

I repeat running the agarose gel electropheresis and adding the denaturation steps, and I get very nice bands. Thank you so much!

Thank you for updating, @  Nadhirah Ahmad.

Hi Nad. That's great. RNA has a lot of secondary structure so you should routinely denature as described

congratulation for getting nice bands.