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Hello, I extracted RNA from human cancer cell line, and I run agarose gel electrophoresis to detect any genomic DNA contaminants in my samples. Here are the conditions: 1% agarose gel in 0.5%...
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Hello all, I ran native agarose gel electrophoresis to check the integrity of my RNA sample. However, I am a little bit confused with the result. I supposed to get only 2 bands, which are 28S and...
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Hello all, What should I do to get rid of non specific band on western blot? 1. I used 5% BSA in TBST to block the membrane (1h, at RT) 2. Polyclonal primary antibody dilution: 1:1000 (overnight,...
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