How do one prepare Disulfiram in olive oil solutions?

I have read several articles mentioning the preparation of the drug disulfiram (DSF) in olive oil for oral gavage of rodents. However, in those papers, there is no description of how this is...

06 January 2021 3,810 1 View

What is the black block for Dark Current in a UV-Vis Spectrometer machine? How to insert it in a Spectrometer machine?

I am trying to construct a calibration curve on a new UV-Vis Spectrometer machine (T80+, PG Instruments Ltd). But when I start by setting the parameters using K-Factor method (pressing...

06 October 2020 2,521 3 View

How a devoted health blockchain could effectively identify and track covid-19 patients while preserving the confidentiality of medical records?

Dear colleagues, I am currently working on a health's blockchain able of integrating informations relating to COVID-19 patients. The difficulty is to allow this blockchain to be a general...

21 April 2020 8,492 4 View

Mammary glands in Platypus ?

Dear researchers, Can anyone help me to identify these odd mammary glands in Ornithorynchus anaticus ? I struggle to understand its mecanism : The secretion from all these flat lobes have a...

03 March 2020 8,275 2 View

AnnexinV-FITC/IP staining for apoptosis detection false positive?

HI, I'm currently working on adherent human epithelial cells. In ordrer to evaluate the apoptosis effect of a drug, the cells need to be cultured in a FCS-free medium before the addition of the...

29 January 2020 3,461 3 View

Abaqus Explicit: difference in TEMP, STRESS and PEEQ computation between adiabatic and coupled ?

Hi, I have the two following INP files for Abaqus where the only difference is that in one case I'm using a CAX4R element on the other case, I'm using a CAX4RT element. Solver has been changed...

05 December 2019 5,803 2 View

Why IV curve of outdoor test is linear/?

Hello, I collected data from outdoor test of organic photo voltaic to analyse stability but when plotted current/voltage i got a curve which is difficult to determine Isc, Voc,FF and PCE..can...

11 September 2019 9,552 2 View

How to connect Solar panels and acs712?

Hello all, Please i want to collect data from solar panels using acs712 current sensor but i am a bit confused. solar panels is connected to dc/dc converter with an output to charge battery. so i...

24 July 2019 6,946 4 View

Anyone knows Solar IV curve tracer software?

Hello Researchers, Kindly I am looking for IV curve tracer software for solar project. could you suggest to me a software for this work? Best regards

21 April 2019 2,600 3 View

Modelling for degradation of organic solar cell?

Hello, I am working on the degradation of organic solar cells, can anyone helps with the equation to be used for modelling purpose in Matlab to plot the IV curve. is it possible to use the same...

18 March 2019 9,170 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View