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Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How to calculate the field capacity??

28 December 2020 1,521 2 View

Which software should be used for XRD analysis in case of soils?

I am using XRD test for indentification of minerals and amount of it.

10 December 2020 8,173 4 View

How to improve total RNA concentration after transfecting the cells with the desired delivery system ?

Hello, I have been doing some transfection experiments for certain gene studies and wants to further do gene expression studies using qRT-PCR. Currently, I am using 7*10,000 cells per well for...

08 December 2020 6,981 5 View

What is the index of germination and howa calculate it??

16 November 2020 4,856 8 View

How do I determine the units of polymerase activity for my enzyme DNA Polymerase?

One unit of DNA polymerase activity is conventionally defined as the amount of enzyme that will incorporate 10 nmol of nucleotides during a 30-min incubation. I found out about the...

04 November 2020 8,824 7 View

How to find the coordinate location of shared information in Google Drive?

Is it possible to know the coordinates of User Equipment which sent the data/information/file to another User Equipment on Google drive? Does drive has such features or option to identify the...

23 August 2020 3,315 3 View

Is there any journal who currently accepting the papers on IoT in Agriculture ?

I need information regarding publication of journal papers. The indexing of the journal should be SCI/ SCOPUS or equivalent. The topic of the journal can be IoT or automation in Agriculture or...

30 May 2020 6,794 5 View

Three single phase choke or one three phase choke.. which is better to have in circuit ?

I have 3-4 PWM converters , output is 3 phase 400 V AC 50 Hz. Now I want to connect them in parallel, to increase the output kVA rating. So a decoupling choke is required at the output side to...

30 March 2020 4,525 3 View

Can anyone suggest me a good ligand exchange method for surface modification of nanoparticles ?

I am working on certain nanoparticles where I need to modify their surface with a polymer by Ligand exhcange approach. I came across many papers describing many different methods but I am really...

07 October 2019 5,174 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View