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Questions related from Richa Khanduri
Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...
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Do aptamers require special equipment to generate?
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One unit of DNA polymerase activity is conventionally defined as the amount of enzyme that will incorporate 10 nmol of nucleotides during a 30-min incubation. I found out about the...
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I am in the need of some guidance regarding which exosome/EV marker to test in patient samples. I tested CD63, CD9 & CD81 in EVs derived from healthy serum and patient serum by FlowCytometry....
24 October 2016 2,641 2 View
I am working with breast cancer cells. I recently got mycoplasma contamination . I checked all my stocks and discarded the mycoplasma positive stocks and expanded/ froze the mycoplasma negative...
17 July 2016 3,386 10 View
I prepared sample for SDS PAGE from serum in reducing conditions using Laemmli buffer and heating it for 10 min at 95 degrees. But due to aggregation I couldnot load the sample on SDS PAGE...
07 January 2015 5,943 7 View
I get a lot of background on my blot. Probably its due to albumin and other lipoproteins in blood. Is there any way to get rid of them?
01 December 2014 446 3 View
