Are there any CHO genome databases with genomic data on >10 CHO cell lines?

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Dialysis tubing vs Protein concentrators?

I am looking into doing a peptide conjugation and the protocol im looking at says to use dialysis tubing to separate the reacted peptide from unreacted. I do not have much of the peptide so i...

09 October 2019 5,695 3 View

Quick isolation of basic peak on an antibody?

Im working on an antibody that has a strange basic peak that does not come down after CpB treatment. After treatment, the peak averages at 5%. I attached pictures below. Mainly interested in the...

11 December 2018 5,730 2 View

Anyone have a trans-membrane TNF alpha expressing mammalian cell line?

Anyone have a trans-membrane TNF alpha expressing mammalian cell line that you would be willing to share a couple vials of? Just need it for a FACS binding assay. I cant find any for sale online,...

08 September 2018 5,866 1 View

Is there a method to purify small amount of DNA at high concentration?

Hi all, I need to screen a random library of genetic elements in mammalian cells after cloning, so I need to DNA purify >50 constructs. I understand that the concentration of DNA from a...

05 June 2018 1,589 7 View

How to enhance mammalian cell growth rate through media additions?

I need to reduce the length of my cell culture process. I was thinking of doing this by shifting log phase earlier on, but I cant find any papers that have enhanced cell growth through media as of...

04 May 2018 2,718 4 View

Are there any ~1 nm size fluorescent particles?

Or what is the smallest known fluorescent molecule?

11 December 2017 8,416 3 View

How to reduce mammalian cell culture length?

My lab is working with a novel CHO cell line adapted from CHO-K1. We would like to use it for production however, its culture lengths are 20+ days. We tried to reduce culture length by starting...

08 September 2017 6,224 5 View

What is the lowest level life form with highest complexity immune system?

Are there bacteria or fungi with immune systems to attack neighboring foreign cells? Do insects exhibit GVHD (can they distinguish their own cells from another insect of the same species)?

08 September 2017 2,205 8 View

How to maximize ssDNA flowthrough PDMS?

I would like to perform an experiment that is dependent on ssDNA successfully flowing out of a PDMS microchannel for collection. However, ssDNA adsorbs onto hydrophobic surfaces, and PDMS is...

07 August 2017 7,918 0 View

Silanization dryness conditions?

Is 200 proof Molecular biology grade ethanol dry enough for usage with APTES for silanization of glass? And what kind of results would i get if I perform the silanization in a normal chemical hood...

07 August 2017 8,386 3 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View