Dialysis tubing vs Protein concentrators?

I am looking into doing a peptide conjugation and the protocol im looking at says to use dialysis tubing to separate the reacted peptide from unreacted. I do not have much of the peptide so i...

09 October 2019 5,555 3 View

Quick isolation of basic peak on an antibody?

Im working on an antibody that has a strange basic peak that does not come down after CpB treatment. After treatment, the peak averages at 5%. I attached pictures below. Mainly interested in the...

11 December 2018 5,634 2 View

Anyone have a trans-membrane TNF alpha expressing mammalian cell line?

Anyone have a trans-membrane TNF alpha expressing mammalian cell line that you would be willing to share a couple vials of? Just need it for a FACS binding assay. I cant find any for sale online,...

08 September 2018 5,748 1 View

Is there a method to purify small amount of DNA at high concentration?

Hi all, I need to screen a random library of genetic elements in mammalian cells after cloning, so I need to DNA purify >50 constructs. I understand that the concentration of DNA from a...

05 June 2018 1,494 7 View

How to enhance mammalian cell growth rate through media additions?

I need to reduce the length of my cell culture process. I was thinking of doing this by shifting log phase earlier on, but I cant find any papers that have enhanced cell growth through media as of...

04 May 2018 2,610 4 View

Are there any ~1 nm size fluorescent particles?

Or what is the smallest known fluorescent molecule?

11 December 2017 8,321 3 View

How to reduce mammalian cell culture length?

My lab is working with a novel CHO cell line adapted from CHO-K1. We would like to use it for production however, its culture lengths are 20+ days. We tried to reduce culture length by starting...

08 September 2017 6,125 5 View

What is the lowest level life form with highest complexity immune system?

Are there bacteria or fungi with immune systems to attack neighboring foreign cells? Do insects exhibit GVHD (can they distinguish their own cells from another insect of the same species)?

08 September 2017 2,112 8 View

Silanization dryness conditions?

Is 200 proof Molecular biology grade ethanol dry enough for usage with APTES for silanization of glass? And what kind of results would i get if I perform the silanization in a normal chemical hood...

07 August 2017 8,273 3 View

How to maximize ssDNA flowthrough PDMS?

I would like to perform an experiment that is dependent on ssDNA successfully flowing out of a PDMS microchannel for collection. However, ssDNA adsorbs onto hydrophobic surfaces, and PDMS is...

07 August 2017 7,803 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View