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Questions related from Nikhil Goel
I am looking into doing a peptide conjugation and the protocol im looking at says to use dialysis tubing to separate the reacted peptide from unreacted. I do not have much of the peptide so i...
10 October 2019 5,655 3 View
Im working on an antibody that has a strange basic peak that does not come down after CpB treatment. After treatment, the peak averages at 5%. I attached pictures below. Mainly interested in the...
12 December 2018 5,701 2 View
Anyone have a trans-membrane TNF alpha expressing mammalian cell line that you would be willing to share a couple vials of? Just need it for a FACS binding assay. I cant find any for sale online,...
09 September 2018 5,826 1 View
Hi all, I need to screen a random library of genetic elements in mammalian cells after cloning, so I need to DNA purify >50 constructs. I understand that the concentration of DNA from a...
06 June 2018 1,559 7 View
I need to reduce the length of my cell culture process. I was thinking of doing this by shifting log phase earlier on, but I cant find any papers that have enhanced cell growth through media as of...
05 May 2018 2,687 4 View
I just keep finding data on CHOK1, CHOK, DG44, DXB11. I was hoping I could get access to quite a few CHO genomes to make genomic fingerprint comparison easy. My backup plan is to use mouse cell...
04 April 2018 7,365 3 View
Or what is the smallest known fluorescent molecule?
12 December 2017 8,390 3 View
My lab is working with a novel CHO cell line adapted from CHO-K1. We would like to use it for production however, its culture lengths are 20+ days. We tried to reduce culture length by starting...
09 September 2017 6,191 5 View
Are there bacteria or fungi with immune systems to attack neighboring foreign cells? Do insects exhibit GVHD (can they distinguish their own cells from another insect of the same species)?
09 September 2017 2,178 8 View
I would like to perform an experiment that is dependent on ssDNA successfully flowing out of a PDMS microchannel for collection. However, ssDNA adsorbs onto hydrophobic surfaces, and PDMS is...
08 August 2017 7,882 0 View
Is 200 proof Molecular biology grade ethanol dry enough for usage with APTES for silanization of glass? And what kind of results would i get if I perform the silanization in a normal chemical hood...
08 August 2017 8,359 3 View
Looking to see if anyone can spare a few vials of an immortalized adipocyte cell line. Doesn't really matter if its white, brown or beige or if its preadipo or adipo. hoping for human but mouse or...
01 January 1970 1,648 0 View
Im thinking about doing some work with >80kb plasmids and I wanted to get a better understanding for working with such large vectors. It seems like BAC is the only way to go and pBeloBAC11 is...
01 January 1970 4,780 3 View
Im new to data science, and was wondering if someone could point me in the right direction to start understanding how to perform the correct statistical analysis/decomposition/modeling analysis of...
01 January 1970 4,435 7 View
