Hi Friends
I am studying plasmonic photothermal damage effect on cancer (DLD-1) and healthy (Fibroblast) cells. During laser irradiation apoptosis and necrosis indexes of cancer cells exhibited more amounts compared with normal cells. Has anyone got some references similar to this case?
Any help will be appreciated.
sincerely yours
Behzad
Dear Behzad,
The attached review article covers the answer to your question. I have copied an important for quick view:
The use of nanosecond pulsed laser for PTT is highly selective and localized damage controllable from few nanometers to tens of micrometers depending on the laser pulse duration and particle size [114]. This makes the method useful for single metastatic cell killing and small tumor eradication. However, the heating efficiency is relative low due to heat loss during the single pulse excitation. So the use of CW laser is favorable for effective heat accumulation to induce mild cell killing in a larger area mainly via hyperthermia and possible coagulation and vaporization depending on the heat content. Nonetheless, the treatment using CW lasers is time consuming (minutes) compared to pulsed laser (single pulse time). Examples using CW lasers for PTT includes selective cancer cell killing [108] and targeted macrophage destruction [112]. In the studied by El-Sayed et al. [108], 40 nm An NPs were conjugated to anti-EGFR antibodies and targeted to two types of human head and neck cancer cells. Detected by dark field light scattering and surface plasmon absorption spectra on single cells, the nanoparticles induce cancer cell damage at 19 W/cm2 after the irradiation with a Ar+ laser at 514 nm for 4 min, while healthy cells do not show the loss of cell viability under the same treatment. Further numerical calculation shows temperature rises to 78 °C capable of inducing cell damage.
Hoping this will be helpful,
Rafik
Hi Behzad, I don't know about the specifics of your assay, but some cancer cell lines are very hardy and can withstand more punishment than primary cells.
Also, be aware that cancer cells in vivo are more heterogeneous than in vitro cancer cell lines. This in vivo cancer cell heterogeneity is the reason for therapy resistant cancer.
I agree with Steingrimur
Please specify which cell line, which laser wavelength and which culturing condition you use to be able to help.
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