I need to to pulse some primary T cells with an enzyme that has bacterial lipopolysaccharides (LPS) as a substrate. The enzyme is generated by Antigen presenting cells and secreted to target LPS in order to neutralise it, since LPS can induce an immune response. If I purchase this recombinant enzyme tagged with Histamine and purified from E.coli, will it be active when introduced to cells in culture at 37 degrees Celcius?
The enzyme may be active as purchased, however, the extent of its activity is likely to dependent upon the constituents in the cell culture medium or enzymatic reaction condition. Therefore, you may need some optimization of the reaction conditions before using for your experiment.
Professor Choubey,
Thank you for your response. I will look into the conditions in which this enzyme could potentially function optimally.