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Questions related from Randeep Singh
I am working with lipids and wanted to know whether there is a fatty acid quantification kit that can measure the concentration of free fatty acid in solution? I have come across one from Abnova,...
14 December 2017 3,463 5 View
How many cells would be sufficient to extract cytosolic protein for western blot analysis? For instance between 1 x 10^6 - 2 x 10^6 cells?
03 December 2017 7,188 4 View
There seems to be a variety of E.coli LPS available from companies such as Sigma. I wanted to pulse primary monocytes and T cells with LPS in culture. What should I be looking for when purchasing...
28 November 2017 9,600 2 View
For protein extraction I am using the Roche cOmplete Tablets , Mini EASYpack. The instructions suggest dissolving one table in 10 ml in H2O or phosphate buffer. However, it does not mention how...
28 November 2017 8,589 7 View
I need to to pulse some primary T cells with an enzyme that has bacterial lipopolysaccharides (LPS) as a substrate. The enzyme is generated by Antigen presenting cells and secreted to target LPS...
15 November 2017 8,441 2 View