Randeep Singh

5 Questions 6 Answers 0 Followers

Questions related from Randeep Singh

Free fatty acid test kits?

I am working with lipids and wanted to know whether there is a fatty acid quantification kit that can measure the concentration of free fatty acid in solution? I have come across one from Abnova,...

14 December 2017 3,463 5 View

No. of cells for cytosolic protein extraction in Western Blot?

How many cells would be sufficient to extract cytosolic protein for western blot analysis? For instance between 1 x 10^6 - 2 x 10^6 cells?

03 December 2017 7,190 4 View

Which Lipopolysaccharide (LPS) to purchase?

There seems to be a variety of E.coli LPS available from companies such as Sigma. I wanted to pulse primary monocytes and T cells with LPS in culture. What should I be looking for when purchasing...

28 November 2017 9,600 2 View

Volume (or concentration) of protease inhibitors to add?

For protein extraction I am using the Roche cOmplete Tablets , Mini EASYpack. The instructions suggest dissolving one table in 10 ml in H2O or phosphate buffer. However, it does not mention how...

28 November 2017 8,590 7 View

Are recombinant enzymes (usually His tagged or GST) purchased in purified form, active when added to cell cultures?

I need to to pulse some primary T cells with an enzyme that has bacterial lipopolysaccharides (LPS) as a substrate. The enzyme is generated by Antigen presenting cells and secreted to target LPS...

15 November 2017 8,442 2 View