I have been working with MCF-7 recently. But I think these cells are different from the MCF-7 cells that I worked with a few years ago. Furthermore, even I don't know what these round cell on the culture are? Are these dividing MCF-7 cells? Sometimes these round cells become very numerous (especially in high confluency) and sometimes they become few. I took some photos from my cells under the microscope at different magnifications and different cell confluences.
Does anyone have any experience with these cells? what are these round cells? Are these MCF-7 cells good for working?
Thank you in advance.
Do the round cells show movement? If it is moved, that means your cells contaminated. If it doesnt move, You dont need to worry because sometimes my MCF-7 also showing round cells while its confluence
Dear Rizky Clarinta Putri,
Thank you for your answer. The round cells don't move. But I think those cells were high passage cells, So I replaced them with other MCF-7 cells. These new MCF-7 is Ok.
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