I am performing CRISPR (WT Cas9 not nickase) for a gene KO on zebrafish. I recently came across a paper which stated that the off-target effects can be eliminated after a few generations of outcrossing and I would like to look further into this. However I cannot locate the paper for the life of me. Does anyone know some good papers on this topic?
Lech Kaczmarczyk
Hi Amanda,
The only off-target mutations not eliminated by subsequent crosses will be the ones linked to the mutated allele(s). There are plenty of papers on that topic, just browse the Pubmed (including methods for detecting those mutations, like this one: 10.1038/nbt.3127).
Cheers,
Lech
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