Amanda Edson

6 Questions 13 Answers 0 Followers

Questions related from Amanda Edson

Has anyone tried making a rotenone stock solution for treating zebrafish embryos?

I would like to treat zebrafish embryos with nM concentrations of rotenone however it is hard to accurately measure such small amounts of the dry powder. Has anyone tried making stock solutions in...

20 February 2019 8,575 0 View

How do I link Endnote lists for Tables and Manuscript?

I am writing a paper and because my tables are large the journal has asked I submit them as separate word documents rather than placing them in the manuscript file. I need to have references in my...

18 December 2018 434 3 View

Are CRISPR off target effects eliminated by out-crossing?

I am performing CRISPR (WT Cas9 not nickase) for a gene KO on zebrafish. I recently came across a paper which stated that the off-target effects can be eliminated after a few generations of...

09 March 2016 1,755 1 View

How do I separate homozygous from heterozygous after CRISPR?

I am performing a KO of my gene by frameshift in zebrafish. I have been screening this by the disruption of a restriction enzyme site in a PCR fragment. I now have an incross of 2 mutant fish and...

29 February 2016 3,366 3 View

What is the best way to do a Western blot on a single zebrafish embryo (5dpf)?

I am using the CRISPR/Cas system and will have zebrafish embryos with introduced indels in my gene of interest. I have a really good antibody for my gene so I would like to western blot some...

12 August 2015 4,035 4 View

Are there experimental tests for off target effects in CRISPR?

I am pretty sure the only way to find off target effects in CRISPR is through genomic sequencing but just in case. Does anyone know of any experimental methods, once you have produced your...

20 July 2015 1,038 5 View