ARA method for free living in soil?

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How many reads are "sufficient" to characterize free living nitrogen fixation microbial communities in soil?

I plan to do nifH targeted NGS (most likely paired end reads with MiSeq) to study free living nitrogen microbial communities in different tillage management system soil samples. I see as much as...

09 October 2017 1,773 1 View

15N2 method for Nitrogen fixation and would like to share your experience?

Hi, We are working on 15N2 method to determine Nitrogen fixation by free living in Soil. However, the difference in between samples of natural abundance (NA) and Enriched (EN) by 15N2 is very very...

09 October 2017 4,814 3 View

Does incubation period influence 15N2 isotope results

Hi, I tried 15N2 method to determine free living bacterial activity in soil samples at 2d, 4d and 8d incubation period. However, i have did not find considerable activity. Does incubation period...

06 July 2017 3,186 4 View

Appropriate primers set for nifH?

Hi, Could you suggest me the primers set for nifH that can cover the good sequence variability of the nifH gene and capture the full diversity. Thanks

04 May 2017 6,419 2 View

How to Convert ul/l to umol/g?

Please let me know how to convert ul/l to umol/gram gas samples for 1000mL jar volume. Thanks..

31 December 2016 1,640 0 View

Determine nitrogen fixation by free living in soil?

Hi, I spent lots of time to figure out ARA method to determine nitrogen fixation by free living in soil. However, I am not getting significance difference between ethylene peaks of control (no...

10 November 2016 6,832 3 View

How to calculate ul/l C2H4 in per gram Soil?

Hello, I need to calculate ul/l C2H4 in per gram soil. For instance, I got 7.098ul/l C2H4 in 1040mL jar at 250g of Soil. How to calculate this C2H4 in per gram soil.

10 November 2016 8,299 0 View

Does soil became Anaerobic in 15 days long incubation?

Please advise, I need to keep my soil sample for long incubation (15 days) to determine nitrogen fixing using acetylene reductase method. In between does any chance to became soil Anaerobic.

09 October 2016 8,033 2 View

Mothur data error?

test.trim.fasta does not showing any data content while using Mothur. Please advice..

09 October 2016 7,736 2 View

How to prepare soil samples for DNA Isolation?

Hi, I will use PowerSoil DNA Isolation Kit for bacterial study. I wanted to know before use kit how to prepare soil samples. Currently, these are frozen in -20C. how long soil should keep on room...

09 October 2016 4,206 1 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View