Aptamer can be free at 5 ' end or 3' end does that affects its binding with the target receptor..??

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Alexander Sasha Vlassov

it depends. for some aptamers one can modify 3' or 5' or both termini- and it will still bind the target well. but sometimes (depending on the aptamer, target, and nature of terminal modification) performance is compromised

Vishakha Tambe

Thankyou Alexander. Your answer has really help me a lot to clear my doubt regarding aptamer binding.