Vishakha Tambe

4 Questions 5 Answers 0 Followers

Questions related from Vishakha Tambe

Did anyone transfect HAP1 cells with plasmid larger size around 8.5 kb. My transfection efficinecy is very low Just three to four cells...??

I tried transfecting HAP1 cells with electroporation programme by Lonza (X005,Y007,X007 and X001). Our plasmid size is 8.5 kb. Positive control plasmid eGFP is around 3.5 kb and that shows good...

12 October 2023 8,660 1 View

Molecular weight determination by GPC of polymer having MW of 50 kDa and purity is 95 % ....??? We are getting Mn, Mw valuus which are 1000 times MW.?

We are using GPC to determine MW of polymer. we are using multidetector analysis however we are getting Mw, Mn and other values o=for polymer 1000 times more than that of actual molecular weight....

08 September 2020 7,103 6 View

Aptamer can be free at 5 ' end or 3' end does that affects its binding with the target receptor..??

Aptamer binding is based on their conformational structure. Does the free 5' end or 3' end affects aptamer binding with the target receptor.. .???

01 March 2018 5,172 2 View

Does molecular weight of hyaluronic acid effects size of the nanoparticle synthesized from it ...???

We are preparing hyaluronic acid ( Molecular weight 50 kda) nanoparticles by desolvation process but getting higher size of 800 nm. Can anyone suggest alternative to get lower particle size of...

20 February 2018 3,516 1 View