Anyone working with HL60 cells?

More Devina Lobine's questions See All

What are the steps and kits to be used post immunoprecipitation of protein lysate and prior to sending it for MS so that there is a clean signal?

I have immunoprecipitated my protein samples with a specific antibody and we then want to send it out for Mass spec analysis to understand its interactomes. However I read that the antibody, salts...

01 June 2023 7,606 0 View

How dashboard is useful in analysis of data ?

How to deal with quantitative data? Which methods are there for data analysis?

05 March 2023 3,315 4 View

Is it possible to obtain more than 100% FE for hydrogen production from CO2 electroreduction ?

I'm working on a catalyst to convert CO2 to CO electrochemically. However, with the current condition I'm using (alkaline flow cell, KOH 1 M electrolyte, and carbon based catalyst), I obtain very...

28 November 2021 6,494 6 View

Why did firm size and firm age often used as control variable in accounting researches?

I got a recommendation about using firm size and firm age as a control variable for my research. But I still confused why both variables used as a control variable, meanwhile in my research...

30 April 2021 5,779 4 View

Why manganese salts seems hard to react with certain schiff base?

I'm reacting a schiff base from salicylaldehyde and p-chloroaniline and trying to react it with mangan(II) salts. I've tried mangan(II) chloride and mangan(II)acetate but both likely produce the...

26 July 2019 4,665 10 View

What can I do to solidify my imine?

Im trying to synthesize imine base from salycilaldehyde and naphtylamine using ethyl lactate as solvent.. everytime I tested them on TLC, naphtylamine always show under UV light, and they say...

19 May 2019 545 2 View

What purification method should I use when my synthesis product isn't even separated by any eluent combination in TLC?

I've done a synthesis reaction with 3 precursor. the product has shown different physical appearance compared to those three precursors, but when it comes to TLC, the stain just won't separate by...

02 April 2018 6,258 9 View

Svm classifier as fitness selection from genetic algorithm feature selection

hi friends, i want to ask if you have a matlab code for genetic algorithm feature selection which use svm classifier for fitness selection, i'm using this for intrusion detection system thanks

26 April 2017 5,848 4 View

Does anyone have any tips for digesting agarose gels with papain?

We are currently using the digestion solution below to digest cells and matrix grown within 2% agarose gels however large pieces of agarose still remain. Any suggestions on how to trouble-shoot or...

12 January 2017 1,195 3 View

Can anyone recommend a good assay kit for PDH activity?

Im looking for an assay to measure the PDH activity in cancer cell lines. Has anyone used a PDH activity assay which works well. Thanks in advance!

21 October 2015 5,566 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View