Does anyone have experience with there being no gene by double digestion after cloning?

I'm working on baculovirus expression system (Bac-to-Bac) using Pfast-Bac-1 as a transfer vector , i have cloned my gene (2236) into the vector using SnabI and HindIII Enzymes , and i got positive...

07 July 2013 6,311 1 View