Anyone suggest the easiest method for determination of activity of cytoplasmic and mitochondrial SOD ?

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Christian Q. Scheckhuber

Have a look at the attached publication. It can help you with setting up your experiments.

Cheers,

Christian

Article Measurement of superoxide dismutase, catalase and glutathion...

Christine Weydert

The in-gel activity assay is a great visual method. You'll need to do the a quantitative activity assay as a follow-up. Everyone will want to see both in any publication.

Manisha Tikka

determination of activity of cytoplasmic and mitochondrial SOD by calorimetric method