Anyone methods in culturing human primary brain endothelial cells in transwells?

More Jijun Dong's questions See All

Can i apply a public questionnaire but analyse it with a different data analysis method?

First, thanks for taking the time to read my puzzle 🥹! I am currently working on the methodology part of my dissertation, and I have chosen a questionnaire as the instrument for my quantitative...

27 July 2024 3,457 3 View

Can anyone help me explain my problem?

Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...

26 July 2024 2,136 0 View

I can't detect any protein in my Western blot. Does anyone know how to fix this?

I'm doing a Western blot and haven't seen any protein for two months. After transferring, the marker is visible, but when I detect the protein once, wash it, and try to detect another protein the...

10 June 2024 6,716 7 View

How can I isolate the desired cell population after making a point mutation by using CRISPR/Cas 9? Are there any ways to increase the rate of HDR?

Now, I am using CRISPR/Cas 9 system to make a point mutation at THBD gene. I am wondering how can I isolate the desired cell population after making a point mutation by using CRISPR/Cas 9? Are...

30 May 2024 9,993 0 View

How can I mark cancer cells that are undergoing mitosis?

Is there a convienient way to mark cancer cells that are undergoing mitosis?

05 May 2024 8,169 4 View

For two Raman peaks belonging to the same irreducible representation, can one be polarized and the other depolarized?

If the depolarization ratio of a Raman peak is less than 0.75, it is a polarized band, and if it is greater than 0.75, it is a depolarized band.

04 May 2024 2,829 0 View

Does anyone can help me recognize what families these cephalopod paralarvae belong to?

I'm trying to identify some paralarvae in the central of Vietnam. I hope some experts can help me address this.

14 April 2024 6,425 0 View

Aspen plus burns correlation?

I need to use burns correlation in rate based setup. But in apsen plus they do not supply burns correlation. Can i make burns correlation using fortran code in calculator block?

30 March 2024 2,647 0 View

The results of the geotextile simulation with abaqus are incorrect?

When I did the Geotextile simulation, I set the elastic modulus, Poisson's ratio and yield stress and plastic deformation for the material as in this article, and added the fracture strain with...

14 March 2024 8,276 3 View

Prestained protein marker for BN-PAGE?

I want to perform a western-blotting experiment after blue-native electrophoresis, but I couldn't find a proper prestained protein ladder to indicate protein size as well as good transfer, so what...

26 February 2024 7,289 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Do You Know Untreated TMJ Affects Your Brain And Entire Body?

04 August 2024 6,282 1 View