I want to perform a western-blotting experiment after blue-native electrophoresis, but I couldn't find a proper prestained protein ladder to indicate protein size as well as good transfer, so what methods are used to determine protein size in those existing papers?
Adam B Shapiro
If you use unstained markers, you can stain the blot temporarily with Ponceau S to locate and mark the positions of the markers before developing the blot.
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