Why is my GOI not expressing after lentiviral transduction despite RNA increase and protein is detected in transfection?

Hi, I'm trying to create a stable overexpression line with EF1a-FlagGOI-IRES-GFP using lentivirus packaged with pxPAX2 and pM2G. After transduction of 293T with 8ug/ml polybrene, I was able to...

29 January 2021 1,878 4 View

Anyone interested in co-authoring a book chapter?

Dear all, I've been invited by an editor to submit a book chapter on COVID-19 (not a predatory publisher) and I'm proposing to write a piece on paediatrics. I am looking for two co-authors who...

07 December 2020 6,247 9 View

Only my PCR primers are not working?

I am working on an undergraduate project within a group. The topic is to identify carbapenamase resistnace gene in K.pneumoniae and E.coli. The target genes are OXA-48, KPC, IMP and VIM. We are...

26 November 2020 6,992 5 View

Uneven poly-d-lysine coating/crystallization?

I've encountered problem with large but scarce PDL crystals and spotted coating after 1hr of 0.02mg/ml PDL in PBS incubation, PBS washed and dried. Previous coating from same PDL stock resulted in...

04 November 2019 3,332 2 View

Machine scheduling with duration dependency.

16 May 2017 1,575 6 View

3-way interaction with a mixed of continuous variable and categorical variable with 3 or more levels?

Has anyone conducted 3-way interaction with a mixed of continuous variable and categorical variable with 3 or more levels? To be exact, it is 3-way interaction between a continuous variable, a...

19 April 2016 8,760 11 View

Can anyone help with protein electron microscopy artefacts?

We are studying a protein that forms tubules in vitro. We have some interesting results from negative staining electron microscopy performed on purified recombinant protein. Can someone please...

29 August 2014 1,791 4 View

Why is there a discrepancy in p-values for different output tables in a mixed model when an interaction term is included (SAS)?

I found that by using Proc Mixed in SAS to run a repeated measure ANOVA, the p-values from the table "Solution for Fixed Effects" are different from the table "Type 3 Tests of Fixed Effects" when...

09 June 2014 9,895 3 View

Difference between ordinal and scale in SPSS

Is there any difference in SPSS to specify a variable as ordinal or scale? I know they are different in meaning, but it seems SPSS treat them the same in calculation.

20 February 2012 5,277 26 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

No title

02 March 2021 3,060 3 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

What does Servant Leadership mean today and in the future? What are the advantages and disadvantages?

In current time of many changes in business and world of work, a lot is said and reported about the importance, skills and benefits of leadership. Are soft skills becoming more and more important?...

02 March 2021 8,149 3 View