How to keep L-cysteine from converting to cystine?

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Sascha Rohn

You can try to use ascorbic acid.

Adam B Shapiro

Exclude oxygen by keeping it under nitrogen or argon, and include a metal chelator such as EDTA to inhibit trace metal-catalyzed oxidation.

I've been working with a highly concentrated solution of N-acetylcysteine in water lately, and I haven't noticed any significant oxidation even though the solution is exposed to air and nothing is added to it. I keep it on ice when in use and at -80oC between experiments.

It would be worthwhile to consider what level of oxidation to cystine would be acceptable.