There are two vectors, the dimension of which is 500 or higher.
What method can be used to judge the similarity between the two vectors more reliably? Euclidean distance? Cosine similarity? L1 norm? and so on.
Hello :) I'm studing an unknown gene(about 4kb) of Arabidopsis thaliana. To observe the phenotype when the gene was overexpressed in plants, the pBI vector (35S-unknown gene-GFP) was transformed...
21 April 2024 3,966 4 View
Hello, I am conducting a gibson assembly with 2 inserts and 1 vector. vector size = 13kb insert A size = 1.7kb insert B size = 4.2kb So, I tried gibson with 1:3:3 (vector:A:B) molar ratio. Is this...
26 March 2024 4,455 1 View
Hi! I am trying to analyze a moderated mediation using mplus. I first started with a simple mediation, so X -> M -> Y. Path a (X -> M) is not significant, while Path b (M -> Y) is...
25 January 2024 3,738 2 View
How to keep L-cysteine from converting to cystine? Even at low pH. Are there any reducing agent may work? better FDA allowed
25 December 2023 4,383 2 View
Which journal is or submitting my wave energy research paper?k and far-field motion in wave energy.
13 December 2023 1,525 1 View
Hi, The paper "Robust actions for improving supply chain resilience and viability" has been published twice in researchgate. I would like to know how merge them? Thank you in advance. Best...
28 September 2023 7,015 2 View
Teaching methods of experiential activities in teaching history
27 September 2023 2,792 3 View
So im looking through KEGG pathways and the plan was that, if I can compare the different versions of the same pathway from different organisms, i can have a better understanding of the origin of...
06 July 2023 1,531 0 View
I want to use the hydrodynamic and thermaldynamic model to simulate the self heating effect in SOI FET, and this requires setting SurfaceResistance in the Thermomode. How should the value of...
10 May 2023 5,581 1 View
I would like to ask the following points: 1. Should I use Thermodynamic Model or Hydrodynamic Model for SHE simulation? 2.Is the Drift-Diffusion Model used without considering SHE? 3. May I ask...
21 April 2023 9,545 0 View
Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You
02 August 2024 3,615 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I've been trying to clone my N-terminal inserts in the comercial pEGFP-N1 vector. Initially, I cloned my N-terminal insert into a pGEMT-easy vector to ensure that the insert digestion was done...
09 July 2024 4,277 1 View
Hello, recently i am an issue swith cloning where I am using the subclone method to insert my gene length is 1.5kb and my vector(pMAL-c6T) is 5kb I got the colonies which i screened through the...
04 July 2024 7,977 2 View
I am trying to clone a gRNA (23 bases) into the Crispr cas9 pDirect23A vector by using restriction enzyme cloning. I have performed a complete experiment, starting with plasmid isolation,...
02 July 2024 5,448 2 View
I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...
02 July 2024 5,332 3 View
Hello, I am working on creating a mutant endothelial cell line having Neomycin knock-in present, I have designed my homology arms of 1kb size having neomycin in between them, I would like to...
25 June 2024 1,197 0 View
I am working on a project that involves analyzing remote sensing (RS) images. I need to convert these images into vector data for further analysis. I am looking for automated methods or software...
22 June 2024 6,165 2 View
In my work, Hygromycin B resistance gene is require for screening. I tried to amplify this gene from the vector pIJ941 but failed. I will be thankful, if anyone help me out from this problem by...
19 June 2024 7,822 3 View
Should we use special binary vectors when using plasmid rescue method to detect transgene integration site in plant? that is, there is often no ori site between LB and RB region of simple binary...
28 May 2024 1,873 0 View