For the isolation of endophytic bacteria, the tissue extract can be diluted in an aqueous solution (0.9 % NaCl) and plated on five 10% TSA plates for each dilution (10–1 and 10–2). The plates can be incubated for up to 15 days at 28ºC. Colonies can be selected on days 2, 5, 10, and 15 of incubation and purified in 10% TSA. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769033/

Check https://www.researchgate.net/publication/272379323_Isolation_and_screening_of_endophytic_fungi_from_three_plants_used_in_traditional_medicine_in_Nigeria_for_antimicrobial_activity

Hi dear Jonathan Tordilla

please check the attached file.

Chinaza Godswill Awuchi Shahriyar Valizadeh thank you so much!

DOI:10.1007/s13596-019-00410-z