Jonathan Tordilla

9 Questions 9 Answers 0 Followers

Questions related from Jonathan Tordilla

How to prevent the "edge effect" in 96-well microplates?

Hi all, I've been using the brand: Costar, for a 96-well microtiter plate. Used all columns and rows, and yes the wells of the plate near the edge have lesser volume compared to the wells going...

28 January 2025 1,588 1 View

How to compute for % bacterial growth inhibition using TTC dye for broth microdilution asay?

I will be using Triphenyl Tetrazolium Chloride (TTC) dye for detecting metabolic activity of bacterial cells after incubation in antibiotic treated wells (96-well microtiter plate). I'm aware...

17 January 2025 8,118 1 View

Can I still isolate Actinobacteria (i.e. Streptomyces) from 4 year old stored soil samples?

We have stored soil samples here in the refrigerator (temp: 2°C - 8°C) for approximately 4 years ago, I was wondering if I can still use them with hopes to isolate Actinobacteria. I was preferring...

13 December 2024 8,480 4 View

Can I use MTT dye instead of resazurin for MIC determination via broth microdilution assay?

Hi! Can I use MTT dye instead of resazurin for MIC determination via broth microdilution assay? If yes, what would be the best concentration to reduce interference of the MTT dye with the...

12 December 2024 5,347 2 View

Will 0.1% DMSO dissolve Amphoterin B?

Hi! I will be using Amphotericin B as a positive control for disc diffusion assay (DDA) towards C. albicans, in SD Agar. I need to inoculate 25µg/20μL of Amphotericin B in each disc. I aim to...

22 October 2024 7,223 2 View

Lyophilization of leaves, stem bark, fruits?

Is it recommended to freeze dry harvested leaves, stem bark, and fruits prior to trituration and extraction? I'll use the extracts for phytochemical analysis & in vitro bioassays. Thank you...

01 April 2024 5,963 4 View

Is there a difference between the two reference standards for Thin Layer Chromatography (HPTLC)?

Hi! I'm currently working in thin layer chromatography of some plant extracts for Camptothecin (CPT) content. Which of the two standards should I procure? (S)-(+)-Camptothecin ≥90% (HPLC),...

23 March 2023 8,478 1 View

Anyone here who can share protocols about Endophytic Microorganism/s Isolation from plants?

Any input will be appreciated. Thank you!

31 May 2021 7,102 5 View

Any suggestions for the specs (specifications) of a laptop/desktop computer that can be used for Molecular Docking Tools/Software?

I'm a researcher that will be using USCF Chimera, Autodock, and Autodock Vina tools. I've been using an Intel i5 8th Gen processor (i5-8250U) lenovo E480 thinkpad with 8GB of RAM, and I'm planning...

19 November 2020 3,945 3 View