How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Who can i take a smear from inhibition zone?

I used alcoholic extract of peganum harmala seeds as inhibitor to staphylococcus enterotoxin type A gene (SEA). By using diffusion method I need to know how can i take a smear from zone of...

29 November 2020 3,165 3 View

Preparation of the mix for Standard PCR (without using a kit)?

Hi, Please anyone already prepared the mix for Standard PCR? But without using a commercial kit. It is for staphylococcal enterotoxins genes. I am looking for the concentrations of each...

08 February 2020 4,487 6 View

Preparation of the mix for Standard PCR (without using a kit)?

Hi, Please anyone already prepared the mix for Standard PCR? But without using a commercial kit. It is for staphylococcal enterotoxins genes. I am looking for the concentrations of each...

01 February 2020 512 8 View

What PCR I can use to detect toxic genes from Salmonella sp and E. coli?

Specifically, I want to detect Salmonella enterotoxin gene and E. coli heat stable enterotoxin gene. However, I am confuse which PCR that I can use to detect those toxic genes. What primers should...

04 May 2018 6,395 1 View

Is it possible for epithelial cells to detach from the tissue in an E-cadherin cleavage independent manner?

I'm examining mouse intestines by E-cadherin IHC to determine the effects of an enterotoxin on intestinal integrity. I was surprised to find that the outer layer of rounded cells that have...

09 October 2016 1,302 3 View

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I have used nanoparticles to detect this toxic lectin and would like to know whether my detection limit is sufficient for practical applications.

01 February 2015 8,034 1 View

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09 October 2014 5,593 1 View

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06 October 2014 7,271 1 View

What rapid detection kit for staphylococcal enterotoxins, you advise me to use?

01 January 1970 1,876 4 View

What other methods are available for testing enterotoxiginicity apart from PCR?

I'm working on enterotoxin production potential in S. aureus.

01 January 1970 7,841 2 View