I will use SEA as a positive control for T-cell activation (I stimulate PBMCs).
Has anyone experience from working with S9399 "Staphylococcal enterotoxin A from Staphylococcus aureus" provided by Sigma- Aldrich?
According to Tech service at Merck they do not recommend stor the toxins in aqueous at -20.
Since we will only use a small volume of the product per experiment, and we want to be able to store the product for future experiments and preferably in aliquots .
How have you stored this product, is there a good protocol out there?
Thank you !
Thank you for your help. However, that would be the case if we grew the bacteria in our lab. But we do not grow the bacteria our self, we buy Enterotoxins that are commercially manufactured by Sigma..
Anna Bergerus
Hello Anna,
Since you are going to work with purified enterotoxin, It should be maintained at colder temperatures below -20 degree celsius.
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